Xidhmadan waxa loo isticmaalaa in vitro qualitative nucleic acid lagu ogaado Virus Monkeypox iyo badhtamaha/galbeedka Afrika clade ee serum bini'aadamka, muunadaha dheecaanka nabarrada iyo shaybaar qolofta.Qalabka aasaasiga ah iyo baaritaanka FAM-ku summadan waxaa loogu talagalay in si gaar ah loo ogaado Virus-ka Monkeybux, baaritaanka ROX ee ku calaamadsan bartamaha Afrika, CY5 oo ku calaamadsan clade galbeedka Afrika.Hidde-sidayaasha RNase P ee bini'aadamka ee isla markaa la soo saaray muunadda baadhista ayaa bixisa kontorool gudaha ah si loo ansixiyo habka soo saarista nukleiga iyo hufnaanta reagent.Baaritaanka lagu beegsanayo hiddasidaha RNase P ee bini'aadamka waxaa lagu calaamadeeyay VIC.
| Qaybaha | 48 Tijaabooyin/xirmo | 96 Tijaabooyin/xirmo |
| Dareeraha falcelinta PCR | 672 μL × 1 tube | 672 μL × 2 tube |
| PCR isku dhafka enzyme | 50 μL × 1 tube | 100 μL × 1 tube |
| Xakamaynta togan | 100 μL × 1 tube | 200 μL × 1 tube |
| Xakamaynta xun | 100 μL × 1 tube | 200 μL × 1 tube |
1. Dareenka: 200 nuqul/ml.
2. Gaar ahaan: Wax falcelin ah ma leh Enterovirus(EV), fayraska jadeecada (MV), fayraska Rubella (RV), fayraska Varicella-zoster(VZV), fayraska Dengue (DenV), Human Parvovirus B19(HPVB19), Fayraska Epstein-barr (EBv), fayraska herpes bini'aadamka 6 (HHV-6)
3. Saxsanaanta: CV ≤ 5%.
Nidaamka PCR-waqtiga-dhabta ah: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX Hongshi SLAN -96S/96P, AGS8830, AGS4800