Qalabka waxaa loo isticmaalaa in lagu ogaado tayada tayada leh ee ORF1ab/N/S hiddo-wadaha coronavirus 2019-nCoV ee muunadaha neefsiga oo ay ku jiraan suufka oropharyngeal, suufka sanka, xaakada iyo samaynta isku-beddelka ku qorida HV69-70 del isku mar.Sida lagu soo sheegay xogta GISAID, in ka badan 95% Omicron isku xigxiga kala duwanaanshaha la soo sheegay waxaa ka mid ah tirtirka HV69-70, kaas oo sababi kara fashilka hidda-wadaha S (SGTF) ee qiimaynta PCR.SGTF waxa loo isticmaali karaa calaamade wakiil si loo baadho Omicron.
Qalabka aasaasiga ah iyo baaritaanka FAM sumadda leh waxaa loogu talagalay in si gaar ah loo ogaado hidda-wadaha shaybaadhka ORF ee 2019-nCoV, VIC summeedka baaritaanka N hidda-wadaha 2019-nCoV, ROX summaddiisu tahay S gene HV69-70 del mutation of 2019-nCoV.Hidde-sidayaasha RNase P ee bini'aadamka ee isla markaa la soo saaray muunadda baadhista ayaa bixisa kontorool gudaha ah si loo ansixiyo habka soo saarista nukleiga iyo hufnaanta reagent.Baaritaanka lagu beegsanayo hiddasidaha RNase P ee bini'aadamka waxaa lagu calaamadeeyay CY5.
Nidaamka PCR-waqtiga-dhabta ah: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;