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COVID19-Omicron

Qalabka RT-PCR ee Novel Coronavirus 2019-nCoV

  • Cabbirka: 50 tijaabo/xirmo
  • Qaybaha leh tiro badan oo kala duwan lama wada isticmaali karo.

Faahfaahinta Alaabta

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Sharaxaada Alaabta

Qalabka waxaa loo isticmaalaa in lagu ogaado tayada tayada leh ee ORF1ab/N/S hiddo-wadaha coronavirus 2019-nCoV ee muunadaha neefsiga oo ay ku jiraan suufka oropharyngeal, suufka sanka, xaakada iyo samaynta isku-beddelka ku qorida HV69-70 del isku mar.Sida lagu soo sheegay xogta GISAID, in ka badan 95% Omicron isku xigxiga kala duwanaanshaha la soo sheegay waxaa ka mid ah tirtirka HV69-70, kaas oo sababi kara fashilka hidda-wadaha S (SGTF) ee qiimaynta PCR.SGTF waxa loo isticmaali karaa calaamade wakiil si loo baadho Omicron.

Qalabka aasaasiga ah iyo baaritaanka FAM sumadda leh waxaa loogu talagalay in si gaar ah loo ogaado hidda-wadaha shaybaadhka ORF ee 2019-nCoV, VIC summeedka baaritaanka N hidda-wadaha 2019-nCoV, ROX summaddiisu tahay S gene HV69-70 del mutation of 2019-nCoV.Hidde-sidayaasha RNase P ee bini'aadamka ee isla markaa la soo saaray muunadda baadhista ayaa bixisa kontorool gudaha ah si loo ansixiyo habka soo saarista nukleiga iyo hufnaanta reagent.Baaritaanka lagu beegsanayo hiddasidaha RNase P ee bini'aadamka waxaa lagu calaamadeeyay CY5.

Astaamaha

Saxnaanta

Novel Coronavirus 2019-nCoV nooca duurjoogta ah iyo nooca Omicron (S gene Hv69-70) waxaa lagu ogaan karaa hal baaritaan oo keliya;

Xakamaynta endogenous (Hidoskoobka RdRp) oo lagu daray muunada soo saarista;

Gaar ahaan

Ka-hortagga wasakheynta iyadoo la adeegsanayo UDG (Uracil-DNA Glycosylase System);

Dareenka: 200 nuqul/ml;

Waxtarka

Nidaamka reagent la hagaajiyay, qaybo fudud, natiijooyin degdeg ah 50 daqiiqo gudahood;

La jaanqaadi kara noocyo badan oo qalab PCR ah;

Lagu kalsoonaan karo

Natiijooyinka soo noqnoqda oo la soo saari karo;

Wax fal celin ah ma leh fayrasyada kale ee neefsashada;

Qalabka lagu dabaqi karo

Nidaamka PCR-waqtiga-dhabta ah: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;


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