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COVID-19 NAB (FIA)

COVID-19 Dhexdhexaad ka-hortagga

  • Go'aaminta tirada ee S-RBD Dhexdhexaadinta Antibody
  • (Fluorescence Immunochromatographic Asay)

Faahfaahinta Alaabta

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Tallaalkaagu waxtar ma leeyahay?

SARS-CoV-2 (COVID19) ayaa si xun uga socda adduunka oo dhan, waxaana tallaalka loo aqoonsan yahay habka ugu dhaqaale iyo waxtarka badan ee lagu xakameeyo faafitaanka fayraska.Qiimaynta tallaalka dhaqameed waxay inta badan isticmaasha hababka ogaanshaha antibody-ka ka-hortagga si loo qiimeeyo waxtarka tallaalada iyada oo loo marayo tijaabooyinka dhexdhexaadinta;

Hababka dhaqameedku waa waqti-qaadasho iyo waxtarkoodu hooseeyo, badanaa waxay qaataan 2 ilaa 4 maalmood si ay u dhamaystiraan qiimeynta, sababtoo ah intooda badani waxay isticmaalaan fayrasyada nool, waxay u baahan tahay in lagu sameeyo heerka biosafety 3 ama ka sarreeya shaybaarka, taas oo ah waqti- isticmalaya oo dadaalaya, oo dhib weyn u keenaya qiimaynta fidinta iyo isu geynta.Sidaa darteed, waxaa jirta baahi degdeg ah oo loo helo hab beddel ah oo fudud oo degdeg ah oo ku habboon qiimeynta unugyada difaaca jirka ee dadka tirada badan.

Aehealth COVID19 Dhexdhexaadinta Qalabka Tijaabada Tireed ee Kahortagga Qofka waxa loo isticmaalaa ogaanshaha tirada ee COVID19 ka takhalusida unugyada difaaca jirka ee serum, balaasmaha ama dhiiga oo dhan.Waxa loo isticmaali karaa in si degdeg ah, tiro iyo aad xasaasi u ah loo ogaado in vitro.si kiliinik ah oo loo isticmaalo qiimaynta kaaliyaha ee saamaynta tallaalka cusub ee coronavirus iyo qiimaynta ka takhalusidda unugyada difaaca jirka ee bukaannada soo kabsaday caabuqa ka dib.

Kahortagayaasha Dhexdhexaadinta COVID19 (nAbs)

Dhexdhexaadinta unugyada difaaca jirka waxay si hufan u joojisaa caabuqa iyadoo xannibaysa isdhexgalka ka dhexeeya fayraska COVID19 iyo unugyada martida loo yahay.Inta badan unugyada difaaca jirka waxay ka jawaabaan xayndaabka xidhitaanka reseptors (RBD) ee borotiinka spike, kaas oo si toos ah ugu xidha soo-dhoweeyaha unugyada ACE2.Antibodies-online hadda waxay bixisaa laba unugyada difaaca jirka oo ku salaysan clone CR3022.Halka inta badan S-protein RBD xidhitaanka unugyada difaaca ay u tartamayaan antigen-ku xidhitaanka ACE2, epitope CR3022 kuma xidhna goobta ACE2.

Sidaa darteed kama horjoogsato xidhitaanka unugyada difaaca jirka.Iyadoo CR3022 iskeed u soo bandhigto kaliya saameyn dhexdhexaadin daciif ah, waxaa la muujiyey inay la midowdo unugyada kale ee S-protein RBD ee xira unugyada si looga takhaluso COVID19.

Kahortagayaasha Dhexdhexaadinta COVID19 (nAbs)

Tilmaamaha Tilmaamaha

Hawl fudud

  • Looma baahna xirfadlayaal la tababaro;
  • Baahida muunada hoose, kaliya waxay u baahan tahay 50 μl;
  • La jaanqaadi kara noocyo badan oo shaybaar ah: Serum/Plasma/Dhiigga oo dhan.

Dareen sare

  • Dareenka: 98.95%;
  • Gaar ahaan: 100%.

Wax ku ool ah

  • Waqtiga falcelinta: 15 daqiiqo, Waqtiga tijaabada: 10s;
  • La heli karo, xaalado isticmaalka ballaaran;
  • Baytariga lagu dhex dhisay, in ka badan 200 oo tijaabo ah oo aan lahayn koronto.

Lagu kalsoonaan karo

  • Waxaa lagu xaqiijiyay 3600 oo tijaabo caafimaad ah, 1500 oo tijaabo ah oo laga qaaday muunada qofka cudurka qaba, 900 oo tijaabo ah oo laga sameeyay talaalka, 1200 oo tijaabo ah oo ay sameeyeen dadka caadiga ah;
  • Xogta daawaynta waxaa laga helay tallaalayaasha tallaalka aan firfircoonayn, tallaalka aashitada nukliyeerka, tallaalka borotiinka, iyo dadka fayraska qaba, dadka caadiga ah.
  • Jooji heerka xannibaadda qiimaha ee 30%.
Tilmaamaha Tilmaamaha

Fluorescence Immunochromatographic Principle Assay

Fluorescence Immunochromatographic Principle Assay

Mabda'a aasaasiga ah ee loo isticmaalo xirmadan waa tartanka immunochromatographic. Xariiqda ogaanshaha (T-line) ee xariijinta tijaabada waxaa lagu dahaadhay angiotensin beddelidda enzyme 2 iyo xariiqda xakamaynta (C-line) waxaa lagu dahaadhay anti-bakayle IgG. suufka conjugate waxa lagu dahaadhay ffluorescently summadd leh COVID19 borotiinka RBD iyo ffluorescently calaamadeysan bakayle IgG antibody.Inta lagu jiro ogaanshaha, marka shaybaarku ka kooban yahay antibody-ka la baarayo, isku-xidhka walxaha tijaabada ah ee ku jira muunada iyo antigen-ka fluorescent waxay sameysaa isku-dhafan difaac iyo isku-dhafka difaaca jirka kuma xiri karo angiotensin-beddelaya enzyme 2 oo aan dhaqdhaqaaq lahayn xuubka nitrocellulose. .Xidhiidhiyaha antigen-ka flfluorescent ee aan ku xidhnayn antibody-ka la tijaabinayo ayaa ku xidhi doona angiotensin-ka beddelka enzyme 2 oo aan dhaqdhaqaaq lahayn xuubka nitrocellulose si uu u sameeyo xariiqda ogaanshaha (T).

Codsiga ka hortagga unugyada dhexdhexaadka ah ee COVID19

  • Baaritaanka baaritaanka kahor tallaalka;
  • Natiijooyinka la socodka tallaalka ka dib;
  • Qiimaynta halista infekshanka labaad ee dadka qaba;
  • Qiimaynta khatarta ah ee dadka caadiga ah (oo ay ku jiraan caabuqa asymptomatic) suurtagalnimada infekshanka;
  • Baaritaanka awoodda iska caabbinta fayraska.

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